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Integrated DNA Technologies oligo dt 65
Affinities of dsDNA and oligonucleotides to WT and FITC-modified tctPA and tcuPA in 0.05 m Hepes/NaOH buffer (pH 7.4) at 25 °C

Affinities of dsDNA and oligonucleotides to WT and FITC-modified tctPA and tcuPA in 0.05 m Hepes/NaOH buffer (pH 7.4) at 25 °C

Oligo Dt 65, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligo dt 65/product/Integrated DNA Technologies
Average 94 stars, based on 701 article reviews

oligo dt 65 - by Bioz Stars, 2026-04

94/100 stars

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1) Product Images from "Effects of Extracellular DNA on Plasminogen Activation and Fibrinolysis * "

Article Title: Effects of Extracellular DNA on Plasminogen Activation and Fibrinolysis *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M111.301218

Figure Legend Snippet: Affinities of dsDNA and oligonucleotides to WT and FITC-modified tctPA and tcuPA in 0.05 m Hepes/NaOH buffer (pH 7.4) at 25 °C

Techniques Used:

Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of oligo(dT)65 incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).

Figure Legend Snippet: Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of oligo(dT)65 incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).

Techniques Used: Fluorescence, Incubation, Concentration Assay

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Journal: The Journal of Biological Chemistry

Article Title: Effects of Extracellular DNA on Plasminogen Activation and Fibrinolysis *

doi: 10.1074/jbc.M111.301218

Figure Lengend Snippet: Affinities of dsDNA and oligonucleotides to WT and FITC-modified tctPA and tcuPA in 0.05 m Hepes/NaOH buffer (pH 7.4) at 25 °C

Article Snippet: Custom oligonucleotides (oligo(dT) 20 ; oligo(dT) 65 , oligo(dAT) 10 (a 20-mer containing 10 AT repeats); oligo(dAT) 33 (a 66-mer containing 33 AT repeats); and TEX615-oligo(dAT) 33 , (an oligonucleotide labeled with red wavelength dye TEX615 at the 5′ end) were synthesized by Integrated DNA Technologies Inc. (Iowa City, IA).

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Effects of Extracellular DNA on Plasminogen Activation and Fibrinolysis *

doi: 10.1074/jbc.M111.301218

Figure Lengend Snippet: Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of oligo(dT)65 incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).

Techniques: Fluorescence, Incubation, Concentration Assay

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